Nanotechnology Now - Press Release: Quorum reports on how cryo prep techniques for SEM are being applied in the Laboratory of Food Technology & Engineering at the University of Ghent, Belgium

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Overview of fat bloom development (left side) on a chocolate surface following oil migration: (A1) initial glossy surface; (B1) occurrence of oil blisters; (C1) sprouting of fat crystals from oil blisters; (D1) surface fully covered with fat crystals. Overview of sugar bloom development (right side) on a chocolate surface following moisture migration: (A2) initial glossy surface; (B2) occurrence of blisters comprising sucrose in solution; (C2) sprouting of sucrose crystals from blisters; (D2) surface covered with sucrose crystals.1 (Reproduced with permission from the author, Dr P D Tran)

Abstract:
Quorum Technologies, market and technology leaders in electron microscopy coating and cryogenic preparation products, report on how the Laboratory of Food Technology & Engineering at the University of Ghent, Belgium, applies cryo techniques to prepare food samples for study by scanning electron microscopy.

Quorum reports on how cryo prep techniques for SEM are being applied in the Laboratory of Food Technology & Engineering at the University of Ghent, Belgium

Laughton, UK | Posted on November 7th, 2017

Ir Davy Van de Walle is a senior researcher in the Laboratory of Food Technology & Engineering at the University of Ghent. He works for the head of the laboratory, Professor Koen Dewettinck. Their mission is to design foods on the nano- and microscale which provide the answer to the consumer demand for new products that are tasty, satisfying, healthy, convenient and inexpensive to produce. Moreover, this may create added value to by-products and contribute to overall socio-economic welfare. Put more simply, they want to understand how ingredient interactions and processing affect the quality attributes (texture, mouthfeel and taste among others) of foods (dairy products, margarines/shortenings and chocolate products in particular). The challenge of imaging using scanning electron microscopy (SEM) is the ability to keep the samples in their natural state hence the requirement to employ cryo sample prep and the selection of Quorum's PP3000T system for the task.

Mr Van de Walle describes the background and execution of the imaging process in detail: “Because a field emission gun (FEG) is used in high resolution SEM, it needs to be placed in an ultra-high vacuum of 10-7 to 10-8 Pa. Consequently, these extreme vacuum conditions require that the specimen must not release any gas or vapour when inserted into the SEM. Except for powdered foods such as flour, sugar or milk powder, most foods contain water. Scanning electron microscopes at lower vacuum conditions (Low-Vacuum SEM, Variable Pressure SEM and Environmental SEM) can be used to examine wet, oily and non-conductive samples in their natural state. In this way, there is no need to dry or freeze the specimen. This is particularly useful with fragile food samples. However, cryo-SEM is the most appropriate technique in food research because of its extremely high resolution. The goal of the cryo-SEM is to vitrify the liquid phase with all the constituents, i.e. macromolecules, thus preserving them in their natural and original state. This process is achieved with the use of the Quorum PP3000T cryo sample preparation system. The initial rapid cooling of the sample is the most critical part in the use of the cryo-SEM system. A slush of liquid N2 (-210 °C) is used for the fast freezing to minimize any damage ice crystals may cause. Following, the sample is transferred under vacuum to the cold stage of the preparation chamber, which is mounted on the SEM chamber. The sample can be fractured to expose internal microstructure, etched to reveal greater detail and coated with metal (e.g. Pt) by sputtering. Finally, the sample is transferred into the SEM chamber where it is mounted on a cold stage specifically tailored to the SEM.” Describing the background to choosing the Quorum sample preparation system, Mr Van de Walle said the process followed a tender procedure involving several FEG-SEM vendors. “Several companies (e.g. Jeol and Hitachi) offered their FEG-SEM in combination with the Quorum preparation chamber. During our meetings with these suppliers, they recommended Quorum as being the best performing. We have been very satisfied with our choice and the group has started to publish papers where the cryo prep system has been invaluable. One example is the PhD research1 of Dr Phuong Diem Tran in which the applicability of cryo-SEM for storage stability testing of pralines is reported. These pralines suffered from fat and sugar bloom. These phenomena are illustrated in the images below. Further work including the fat bloom examples have been published in a paper in the European Journal for Lipid Science Technology.2”

Quorum supplies a large range of products and services to meet the needs of electron microscopists. These include sputter & carbon coaters, glow discharge systems, cryo-SEM preparation systems, critical point & freeze dryers for sample preparation, plasma etchers, benchtop vacuum evaporators,

Peltier cooling stages for SEM and more. To obtain full details of Quorum’s range of products and services to support electron microscopists, please visit www.quorumtech.com/systems-and-equipment.

Reference
P D Tran, (2017). Novel strategies to develop filled chocolates for the tropics - PhD thesis, Ghent University, Belgium, 258p.
Controlling the stability of chocolates through the incorporation of soft and hard StOSt-rich fats; P D Tran et al, Eur. J. Lipid Sci. Technol. 2015, 117, 0000–0000, DOI: 10.1002/ejlt.201400584.

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About Quorum Technologies Limited
Quorum Technologies are market and technology leaders in electron microscopy (EM) coating and cryogenic preparation providing high performance sample preparation instrumentation worldwide.

A key feature of Quorum's success is by listening to the industry and being able to respond to requests and requirements to develop new instruments and techniques. This resulted in the introduction of the market-leading Q Series of vacuum coaters and PP3010T cryo preparation system for SEM and FIB/SEM – genuine ground-up redesigns that have earned Quorum the respect of the industry and truly set the standard within the specimen preparation field. The company was founded in 2001 after the acquisition of the long-established Polaron brand and its range of EM products from Thermo Instruments. In 2005, they acquired the Emitech company, allowing Quorum to extend their product range and further grow the business.

Further impetus and investment came with the acquisition of Quorum by Judges Scientific plc and a move to purpose-built factory and offices close to the South Downs National Park at Laughton, East Sussex in the South East of England.

In 2014 Quorum were proud winners of the Queen’s Award for Enterprise for International Trade.

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